Cellular Pharmacology

The tools you need for cell-based assays, mechanism of action studies and biomarker discovery

Tap into the expertise of our scientists to customize your cellular pharmacology assays, whether for routine compound screening in primary and secondary assays, unraveling target mechanism of action, or identifying key biomarkers of response and patient selection. Add depth to your assay development process by harnessing our experience in multiple target classes — including GPCRs, receptor tyrosine kinases (RTKs), ion channels, intracellular kinases and phosphatases, GTPases, polymerases, transcription factors, and splicing factors. With a track record of successfully implemented assays in over 100 cancer cell lines and primary cells, you can rely on our expertise to develop an assay that is robust, reproducible, and accurate.


"I don’t think I can adequately express how grateful I am to have worked with you and your team over the past 1+ year… Having you and the team to talk with, bounce ideas off, and learn from has made all the difference… It is amazing how much data was generated in such a short period of time."

Monitoring cell health: proliferation and cytotoxicity assays

  • Cell growth and viability assays in 96-well or 384-well format
  • Over 100 cancer cell lines in the Paraza Collection — and the list keeps growing
  • Spheroid models in ultra-low attachment (ULA) plates
  • Wide array of read-outs: luminescence (Cell Titer GLO™), absorbance (sulforhodamine B), fluorescence (CyQuant™, Alamar Blue HS™), etc.
  • Culture of primary cells: endothelial, cardiomyocytes, motor neurons, astrocytes, microglia, skin cells, etc.

Homogeneous assays for drug screening

  • Calcium signaling using FLIPR-Tetra® instrumentation
  • Custom-built and off-the-shelf HTRF and AlphaLISA assays
  • NanoBit® or NanoBret® technology
  • Fully licensed to utilize radiolabeled probes (3H, 125I, and 35S)
  • Compound addition using digital dispensers
  • Automated and semi-automated liquid handlers 

Target validation and mechanism of action studies

  • Multicolor quantitative PCR, SNP genotyping or qRT-PCR
  • Customized or off-the-shelf reporter gene assays
  • Western Blot analysis: run >200 samples at once
  • Cytokine quantification by ELISA or bead-arrays (Luminex®)
  • Flow cytometry using 3 or 4 laser, up to 12-color instruments with 96-well plate loader
  • Dose-response matrix analysis of drug combinations using Loewe’s, Bliss and HSA models
  • Proteomics studies, performed in collaboration with DMPK Bioanalysis group

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If you want to learn more about the Paraza difference, contact us to discuss your specific needs.