From protein purification to compound screening, biophysics and everything in between
Harness the full potential of our biochemistry team’s target knowledge and advanced technical skills to develop custom biochemical assays for your primary and secondary in vitro screens. Delve deeper into target characterization, with mechanistic studies such as enzyme kinetics and biophysical assessment of drug-target interactions enabled by state-of-the-art equipment. Take advantage of our protein expression services using E. coli, insect and mammalian expression systems to access multi-step purified, functionally QC’ed recombinant protein. From start to finish, our biochemistry team stands ready at your disposal, ensuring a seamless progression through your early-stage drug discovery campaign.
Client
Testimonial
"You have assembled a truly remarkable team who not only knows how to present science but also knows the science of drug discovery in depth."
Protein expression and purification
- Small-scale (milligram quantity) protein expression services using E. coli, insect and mammalian cell expression systems. Optimization of expression vectors and protein expression conditions for each target.
- Multistep purification using Äkta™ FPLC system: affinity, ion exchange, size exclusion.
- Assessment of purity and functionality to ensure product quality.
Compound screening, hit-to-lead and SAR
- Automation capabilities (Echo, Agilent Bravo, Tecan d300e Nanodispenser, Multidrop™, Apricot, etc.) to handle libraries up to at least 50K
- Screening triage for detection of compounds with non-desirable properties
- Orthogonal validation to confirm hits and flag false positives
- Sensitive and reproducible dose-response evaluations to support SAR development
Customized biochemical assays and biophysics
- Customized biochemical assays using fluorimetric (including FP, HTRF®, and AlphaScreen®) or radiometric (14C, 3H, 125I, 32/33P, and 35S) methods.
- Enzyme kinetics and drug-target interaction studies: determination of affinity, binding kinetics, and thermodynamics
- Binding affinity and kinetics using Surface Plasmon Resonance (SPR) with Biacore™
- Binding thermodynamic parameters using Isothermal Titration Calorimetry (ITC)
- Protein stability and ligand interactions using Differential Scanning Fluorimetry (DSF, thermal shift), among other techniques.
