Tailored assays for better drug discovery
Implementing the appropriate biological assay is pivotal in determining the success of your drug discovery program. Our flexible approach to method development, validation, and implementation guarantees the delivery of the right method for your needs.
With our proven track record working with over fifty clients, you can confidently depend on us to reproduce complex literature methods, enhance them to meet pharmaceutical research standards, optimize existing protocols, and run off-the-shelf assays. The outcome is a test that is both accurate and robust: an assay you can trust.
Client
Testimonial
"The level of engagement, commitment, and dedication to the program that the entire team at Paraza has shown is impressive. The success to date is a reflection of that hard work, and we sincerely appreciate it."
Top-tier expertise integrated into your team
Our approach is built on the depth and breadth of our team’s scientific expertise.
Over 70% of department members hold PhDs, and our multidisciplinary and collaborative ecosystem covers a wide range of expertise across the fields of oncology, metabolic disease, immunology/inflammation, central nervous system (CNS) disease, microbiology, and virology.
We engage with you throughout the process — from ideation, assay design, and implementation to data interpretation and troubleshooting — to help you achieve your goals.
Bioanalytical Solutions
- Quick turnaround for both small-molecule and large-molecule projects
- Bioanalysis of routine in vitro ADME assays and in vivo PK/PD samples from various tissues and biofluids
- In vitro and in vivo metabolite profiling in a variety of media and identification of metabolites using cold material
- Protein characterization and proteomics
- Broad range of more specialized capabilities including custom bioanalytical method development, small biomarker analysis, compound derivatization, and more
Pharmacokinetics (PK) and In Vivo Studies
- Standardized and tailored study designs conducted in rodents and higher species within CCAC accredited facilities
- Single/multiple ascending dose, maximum tolerated dose and acute tolerability studies
- Rat surgical models for drug elimination pathway characterization
- Excretion studies in rodent and non-rodent species
- Formulation development for pre-clinical in vivo studies
In Vitro ADME
- Variety of in vitro assays suitable for both early-stage drug discovery and advanced compound profiling
- Cell permeability assays
- Metabolic stability assessments in microsomes, cytosol, hepatocytes, plasma, blood, and other matrices
- Protein binding measurement by equilibrium dialysis
- Solubility studies at various pHs, including biorelevant buffers
- Specialized procedures such as nuclear receptor assays, transporter studies, and cell viability assays are also available
In Vivo Pharmacology
- Xenograft, allogeneic, orthotopic cancer models
- Inflammation and sepsis models
- Maintenance of mouse colonies
- IVIS in vivo imaging
- Custom optimized formulations
- CCAC accredited facilities
Cellular Pharmacology
- Mechanism of action (M.O.A.) and target validation studies
- Homogeneous assays for drug screening
- Regulation of gene expression, proliferation and cytotoxicity assays
- Gene expression assays
- Cytokine array assays (Luminex®)
- Drug combination studies
- Post-translational modifications
- Primary cells, iPSC and PBMC culture, differentiation and activation
Biochemistry & Biophysics
- Compound Screening, Hit-to-Lead and SAR
- Protein expression and purification
- Customized biochemical assays
- Biophysics
State-of-the art instrumentation
With our continuously growing collection of advanced instrumentation, we can meet your assay needs. Our versatile repertoire enables your research from conducting in-depth protein studies to capturing a comprehensive range of cellular readouts and performing precise in vivo measurements. Explore the representative instruments listed below, or get in touch with us for a detailed description of our comprehensive capabilities.
- LEX Bioreactors (Epiphyte3)
- EmulsiFlex-C5 (Avestin)
- ÄKTA™ pure (GE Healthcare)
- Biacore™ T200 (Cytiva)
- AFFINITY ITC low volume microcalorimeter (Waters)
- Cellaca MX high-throughput fluorescent cell counter (Nexcelom)
- CytoFLEX flow cytometer with 96-well autosampler (Beckman Coulter)
- Attune NxT flow cytometer with 96- and 384-well autosampler (ThermoFisher)
- Luminex® LX200
- Incucyte® S3 live-cell analysis system (Sartorius)
- Cellaca MX high-throughput fluorescent cell counter (Nexcelom)
- ECLIPSE Ti2 fully motorized fluorescent inverted microscope (Nikon)
- BioTek Cytation 5 (Agilent)
- QuantStudio6 and QuantStudio7 qPCR instruments (ThermoFisher)
- 4-D Nucleofector™ (Lonza)
- Qsonica Q800R sonicator (shearing system)
- Jess automated western blot (ProteinSimple)
- Echo 650 Series acoustic liquid handlers (Beckman Coulter)
- D300e Digital Dispenser (Tecan)
- Bravo automated liquid handler (Agilent)
- Apricot S3 liquid handler (sptLabtech)
- Assist Plus with cherry picking capability (Integra)
- Assist Plus with cherry picking capability (Integra)
- EnVision™ 2105 multilabel plate reader with laser-based HTRF (Perkin Elmer)
- ClarioStarPlus (BMG Labtech)
- FLIPR Tetra (Molecular Devices)
- SpectraMax i3/i3x (Molecular Devices)
- Infinite® F200 PRO plate reader (Tecan)
- FlexStation 3 (Molecular Devices)
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